Description
The Enterohemorrhagic E. coli (EHEC) STX2B / Shiga toxin II subunit B ELISA Pair Set is for the quantitative determination of Enterohemorrhagic E. coli (EHEC) STX2B / Shiga toxin II subunit B. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Enterohemorrhagic E. coli (EHEC) STX2B / Shiga toxin II subunit B coated on a 96-well plate. Standards and samples are added to the wells, and any Enterohemorrhagic E. coli (EHEC) STX2B / Shiga toxin II subunit B present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-Enterohemorrhagic E. coli (EHEC) STX2B / Shiga toxin II subunit B monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Enterohemorrhagic E. coli (EHEC) STX2B / Shiga toxin II subunit B present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm